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Faculty of Engineering Science

Biofabrication – Prof. Dr. Leonid Ionov

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Equipment

Our lab counts with all the necessary equipment for polymer processing and analysis, as well as for cell culture studies with several different type of mammalian cells. 

Mechanical/rheological testing

Combined Rheometer | DMA: MCR 702 MultiDrive with controlled temperature (-40°C-200°C) and (20°C-650°C), linear drive for DMA measurements in strain, torsion, three-point bending and cantiliver modes.

Responsible persons: Andrés Posada - Mairon Trujillo

Thermogravimetric Analysis (TGA) 

The TG 209 F3 Tarsus from Netzch is used to obtain knowledge about the thermal stability of polymers and their composition. 

Responsible persons: Pavel Milkin - Muzaffar Maksumov

Differential Scanning Calorimeter (DSC) 

The Mettler Toledo DSC device is used to determine the thermal properties of the processed and synthesized polymers. It measures the enthalpy changes of the samples based on their physical and chemical properties as function of temperature.

Responsible persons: Pavel Milkin - Gissela Constante

Spectroscopic investigations

TriStar² LB 942 Modular Monochromator Multimode Reader with absorbance, fluorescence and luminiscence modes.

Responsible persons: Indra Apsite - Mairon Trujillo

Surface modification

Programmable MiniFlecto®  plasma clear with two gas connections.

Responsible persons: Indra Apsite - Gissela Constante

3D printers

3D Discovery Regen-Hu

3D Discovery printer inside of the biosafety cabinet (RegenHU, Switzerland). It can be used for printing hydrogels, thermopolymers, and melt-electrowrite polymers. It counts with multiple printheads.

Responsible persons: Gissela Constante - Mairon Trujillo

GeeeTech 3D Printer

3D printer based with integrated syringe pump. Syringe and nozzle can be heated upto 100°C. This 3D printer is used to fabricate hydrogel-based 3D structures. 

Responsible persons: Juan Uribe - Amit Shukla

Melt-electrowriting - 3D Printer of Thermoplastic polymers

Dispensing 3D printer for printing of thermoplastics with four heated pneumatic dispensers. Maximum temperature is 350 ° C that allows printing of most commercial and synthetic polymers. Commonly polymers are printed on heated stage (max T = 80°C). Pressure is electronically controlled in the range between 0  and 10 Bar. Printer is equipped with high-voltage power supply (30kV), which allows melt electrospinning writing. 

Responsible persons: Amit Shukla - Gissela Constante

3D Printer of Thermoplastic polymers and Hydrogels

Dispensing 3D printer for printing of thermoplastics/hydrogels with four heated pneumatic dispensers. Maximum temperature is 100 °C. Printing on Peltier-based cooling-heating stage (0°C - 120°C) is possible. Pressure is electronically controlled in the range between 0 Bar and 10 Bar. 

Responsible persons: Amit Shukla - Gissela Constante

Melt-electrowriting - 3D Printer of Thermoplastic polymers

Dispensing 3D printer for printing of thermoplastics/hydrogels with one heated pneumatic dispenser. Maximum temperature is 100 ° C. Pressure is electronically controlled in the range between 0 Bar and 10 Bar. Printer is equipped with high-voltage power supply (30kV), which allows melt electrospinning writing. 

Responsible persons: Arpan Biswas - Aastha Lall

DLP Stereolithography 

DLP(LCD) stereolithography 3D printer Wanhao Duplicator D7 Plus

Responsible person: Indra Apsite

Fiber spinning 

Electro-spinning  

Electrospinning device for simultaneous spinning of two different materials with multiple syringes for mass production. Our e-spinning is equipped with high-voltage power supply (+/-30kV).

Responsible persons: Indra Apsite - Gissela Constante

Extruder  

Noztec Touch Extruder device to fabricate filament from polymer pellets for 3D printing purposes.

Responsible persons: Amit Shukla - Andrés Posada

Touch-spinning

Touch-spinning device used for producing uniaxially oriented fibers from different polymer materials.

Responsible persons: Amit Shukla - Juan Uribe

Chemical lab equipment

Freeze-dryer Labconco

Our lab is equipped for synthesis and modification of polymers including freeze dryer, rotor evaporator, pure water system, vaccum line.

Responsible persons: Juan Uribe - Amit Shukla 

Cell culture laboratory

We own a well-equiped cell culture lab. We make studies of cell compatibility, differentiation and functionality of the biofabricated scaffolds.

Responsible persons: Katharina Bauer - Mairon Trujillo - Arpan Biswas

Fluorescence Microscope 

Fluorescence Microscope Nikon Eclipse Ti2 is provided with incubation system to analize in vivo the cell behavior.

The microscope also counts with a perfect focusing system (PFS).

Responsible persons: Mairon Trujillo - Indra Apsite

Surface characterization

Contact angle 

The contact angle device "Krüss Drop Shape Analyzer - DSA25" is used for wetting investigations and measurement of surface free energy.

Responsible persons: Gissela Constante - Arpan Biswas

Potentiostat

The Interface 1010E and 5000E are general purpose potentiostats that runs basic physical and electroanalytical techniques such as cyclic voltammetry, chronoamperometry, chronopotentiometry, pulse voltammetry and square wave voltammetry.  

Responsible persons: Pavel Milkin - Indra Apsite


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